aggregation of vesicles

Aggregation of phospholipid vesicles by water-soluble polymers

1996/11/1 · Aggregation of large unilamellar vesicles required a lower polymer concentration, probably because aggregation occurs in a secondary minimum (without surface contact). In contrast to experiments in which the polymers were added directlyto the vesicles, dialysis ofthe vesicles against polymer-containing solutions did not induce aggregation.

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Isolation of Extracellular Vesicles: General Methodologies

Background . Extracellular vesicles (EVs) play an essential role in the communication between cells and transport of diagnostically significant molecules. A wide diversity of approaches utilizing different biochemical properties of EVs and a lack of accepted protocols make data interpretation very challenging. Scope of Review.

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PDF) Leakage and aggregation of phospholipid vesicles

Eur. J. Biochem. 268, 48±55 (2001) q FEBS 2001 Leakage and aggregation of phospholipid vesicles induced by the BH3-only Bcl-2 family member, BID Dayong Zhai, Qi Miao, Xiaofeng Xin and Fuyu Yang National Laboratory of Biomacromolecules, Chinese Academy of Sciences, Beijing, China BID is a BH3 domain-only member of the Bcl-2 family that acts as an apoptotic agonist in programmed cell death.

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Synaptic vesicle mimics affect the aggregation of wild-type

Sep 30, 2019 · The aggregation of the A53T mutant, for example, was found to be enhanced in the presence of DMPS (1,2-dimyristoyl-sn-glycero-3-phospho-l-serine) vesicles when compared to the WT, again suggesting differences between the two variants with respect to mechanisms and/or intermediate species formation during aggregation (Flagmeier et al., 2016 ...

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Lipid vesicles affect the aggregation of 4-hydroxy-2-nonenal

Lipid vesicles delayed the aggregation process in a concentration-dependent manner, an effect that was observed only when they were added at the beginning of the aggregation process. Co-aggregation of lipid vesicles with HNE-modified α-syn also induced cytotoxic effects on differentiated SHSY-5Y cells.

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Electrostatic control of spontaneous vesicle aggregation

Aggregation of these vesicles was induced by incorporating a small concentration of biotin-lipid in the bilayer, followed by addition of streptavidin as a cross-linking receptor. Freeze-fracture transmission electron microscopy shows that almost no aggregation occurred in solutions with no added electrolyte, in which the Debye length was much larger than the dimensions of the streptavidin ...

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Aggregation Behavior of Protoporphyrin IX in Aqueous

The aggregation behavior of protoporphyrin IX in aqueous solution, as a function of pH and ionic strength, has been studied by means of UV/vis, fluorescence emission spectroscopy, and resonant light scattering (RLS) techniques. Our experimental results agree with previous literature assignments: (i) protoporphyrin IX is a monomer in the pH range 0−3, (ii) a dimer is present for pH > 8, and ...

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PDF SUPPLEMENTARY INFORMATION Self-limiting aggregation of

Self-limiting aggregation of phospholipid vesicles N. de Lange, F. A. M. Leermakers, J.M. Kleijn January 10, 2020 Figure S1 Vesicle pair modeled as a prolate ellipsoid with axes a, b, b with a>b, in order to estimate its diffusion coefficient. 1Overview of prepared vesicles Vesicle preparation was performed as explained in the materials

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Lipid Vesicle Aggregation Induced by Cooling

Aggregation of lipid vesicles composed of phosphatidylcholines, which have low negative surface charge densities, takes place more readily than vesicles of phosphatidylserines or phosphatidylethanolamines which exhibit greater negative surface charges.

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High-speed centrifugation induces aggregation of extracellular vesicles

2020/12/27 · DOI: 10.3402/jev.v4.29509 Corpus ID: 12447917 High-speed centrifugation induces aggregation of extracellular vesicles @article{Linares2015HighspeedCI, title={High-speed centrifugation induces aggregation of extracellular vesicles}, author={R. Linares and S. Tan ...

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Clathrin assembly protein AP-2 induces aggregation of

The vesicle aggregation reaction can be reconstituted: liposomes of phospholipid composition approximating that found on the cytoplasmic surfaces of the plasma membrane and of coated vesicles (70% L-alpha-phosphatidylethanolamine (type I-A), 15% L-alpha-phosphatidyl-L-serine, and 15% L-alpha-phosphatidylinositol) aggregated after addition of AP ...

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Synaptic vesicle mimics affect the aggregation of wild

The aggregation of the A53T mutant, for example, was found to be enhanced in the presence of DMPS (1,2-dimyristoyl-sn-glycero-3-phospho-l-serine) vesicles when compared to the WT, again suggesting differences between the two variants with respect to mechanisms and/or intermediate species formation during aggregation (Flagmeier et al., 2016 ...

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Lipid vesicles affect the aggregation of 4-hydroxy-2-nonenal-modified α-synuclein oligomers

2018/9/1 · Different modes of aggregation can be triggered depending on the lipid composition, the vesicles curvature and the protein-to-lipid ratio, i.e. the same type of lipid vesicles can either increase or decrease aggregation depending on the relative amount of protein and].

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Vesicle aggregation based on hydrophobic interactions

Aug 27, 2015 · The mechanism of vesicle aggregation was assumed to be due to the temperature-induced configuration transition of a few C m ϕ 2 C m molecules in the vesicles from cis- into trans-form. Thus, a few tails of C m ϕ 2 C m stretched toward the solution. When the vesicles approached, these projecting tails interacted hydrophobically, thereby non ...

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Recent Progress in Fluorescent Vesicles with Aggregation

Jan 21, 2019 · Fluorescent vesicles have recently attracted increasing attention because of their potential applications in bioimaging, diagnostics, and theranostics, for example, in vivo study of the delivery and the distribution of active substances. However, fluorescent vesicles containing conventional organic dyes often suffer from the problem of aggregation-caused quenching (ACQ) of fluorescence ...

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Aggregation of phospholipid vesicles by water-soluble polymers

Water-soluble polymers such as dextran and polyethylene glycol are known to induce aggregation and size growth of phospholipid vesicles. The present study addresses the dependence of these processes on vesicle size and concentration, polymer molecular weight, temperature, and compartmentalization of the vesicles and polymers, using static and dynamic light scattering.

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Cryo-electron microscopy of extracellular vesicles from

2020/1/30 · Extracellular vesicles (EVs) are membrane-enclosed vesicles which play important role for cell communication and physiology. EVs are found in many human biological fluids, including blood, breast milk, urine, cerebrospinal fluid (CSF), ejaculate, saliva etc. These nano-sized vesicles contain proteins, mRNAs, microRNAs, non-coding RNAs and lipids that are derived from producing cells. EVs ...

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Aggregation, fusion, and vesicle formation of modified low density

Initiation of atherosclerosis is characterized by accumulation of aggregates of small lipid droplets and vesicles in the extracellular matrix of the arterial intima. The droplets and vesicles have features that suggest that they are formed from modified plasma-derived low density lipoprotein (LDL) particles.

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The Effect of Alzheimer’s Aβ Aggregation State on the Permeation of Biomimetic Lipid Vesicles

Alzheimer’s disease is characterized by the aggregation and deposition of the Aβ peptide. This 40 or 42 residue peptide is the product of the proteolysis of the amyloid precursor protein membrane protein and is able to assemble to form ordered, stable amyloid fibrils as well as small, soluble, and potentially cytotoxic oligomers. The toxicity of the oligomers may be associated with the ...

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Small molecule-mediated stabilization of vesicle

When αSyn is bound to vesicles, the highly aggregation-prone non-amyloid component (NAC) region (residues 61-95), is in an α-helical conformation and acts as a membrane sensor 25.

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Annexin A1-dependent tethering promotes extracellular

Extracellular vesicles (EVs) including plasma membrane-derived microvesicles and endosomal-derived exosomes aggregate by unknown mechanisms, forming microcalcifications that promote cardiovascular...

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PDF) Tubulation and Aggregation of Spherical Nanoparticles

For the case of multiple rigid spherical nanoparticles adsorbed on vesicles, Monte Carlo simulations demonstrate that these particles could form linear aggregation cooperatively and induce ...

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Trehalose prevents aggregation of exosomes and cryodamage

In contrast, aggregates of independent vesicles were rarely observed suggesting that fusion may be the prevalent mechanism of formation of larger particles. Oval vesicles were observed in areas of...

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Aggregation of Plant Protoplasts by Artificial Lipid Vesicles

Sonicated unilamellar lipid vesicles, consisting of egg lecithin, stearylamine, and cholesterol in 7:2:1 molar ratios, promoted the aggregation of tobacco ( Nicotiana glutinosa ) protoplasts with the aid of mono- or divalent cations. A reaction mixture containing liposomes (0.4 micromoles lipid per milliliter), 50 millimolar CaCl2, 0.5 molar mannitol, and 5 × 105 protoplasts per milliliter ...

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Calcium Assists Dopamine Release by Preventing Aggregation on the Inner Leaflet of Presynaptic Vesicles

The neutrality of the lipid head group region, enhanced by a low pH level, should limit membrane aggregation of transmitters. In addition, the simulations suggest that the high calcium levels inside presynaptic vesicles prevent even the most lipophilic transmitters such as dopamine from adhering to the inner leaflet surface, thus rendering unhindered neurotransmitter release feasible.

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